Biochemical studies on ricin. XVIII. Isolation and characterization of ricin E from castor beans.
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منابع مشابه
Purification and Biochemical Characterisation of Ricin from Castor Seeds
Ricin is a highly toxic plant toxin of Ricinus comtnunis seeds, commonly known as castor seeds. The toxin was extracted and purified using affinity and size exclusion chromatography. The purity of ricin was evaluated by the sodium dodecylsulphate-polyacrylamide gel electrophoresis. Purified ricin gives a single band under non-reduced condition and two bands under reduced condition. The molecula...
متن کاملIsolation of Crystalline Ricin
A toxic crystalline protein has been isolated from crude extracts of castor bean meal. Ultracentrifuge and electrophoresis tests show the crystalline protein to become fairly homogeneous after three or four crystallizations. This is also confirmed by toxicity measurements. Solubility tests, however, indicate the presence of more than one protein component in the crystalline material, possibly i...
متن کاملRicin Toxicity: Clinical and Molecular Aspects
Seeds of the castor bean plant Ricinuscommunis L (CB) contain ricin toxin (RT), one of the most poisonous naturally-occurring substances known. Ricin toxin, a water-soluble glycoprotein that does not partition into the oil extract, is a ribosome-inactivating toxin composed of two chains, labeled A and B. Severity of the toxicity varies depending on the route of exposure to the toxin. Inhalation...
متن کاملHumanization and Characterization of an Anti-Ricin Neutralization Monoclonal Antibody
Ricin is regarded as a high terrorist risk for the public due to its high toxicity and ease of production. Currently, there is no therapeutic or vaccine available against ricin. D9, a murine monoclonal antibody developed previously in our laboratory, can strongly neutralize ricin and is therefore a good candidate for humanization. Humanization of D9 variable regions was achieved by a complement...
متن کاملImmunoglobulin A antibodies against ricin A and B subunits protect epithelial cells from ricin intoxication.
Epithelial cells of the respiratory and gastrointestinal tracts are extremely vulnerable to the cytotoxic effects of ricin, a Shiga-like toxin with ribosome-inactivating properties. While mucosal immunity to ricin correlates with secretory immunoglobulin A (IgA) antibody levels in vivo, the potential of IgA to protect epithelial cells from ricin in vitro has not been examined due to the unavail...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1977
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.41.2041